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Microscale behavior of fluids
Silicone rubber and glass microfluidic devices. Top: a photograph of the devices. Bottom: DIC micrographs of a serpentine channel ~15 m wide.
The behavior of fluids at the microscale can differ from 'macrofluidic' behavior in that factors such as surface tension, energy dissipation, and fluidic resistance start to dominate the system. Microfluidics studies how these behaviors change, and how they can be worked around, or exploited for new uses.
At small scales (channel diameters of around 100 nanometers to several hundred micrometers) some interesting and sometimes unintuitive properties appear. In particular, the Reynolds number (which compares the effect of momentum of a fluid to the effect of viscosity) can become very low. A key consequence of this is that fluids, when side-by-side, do not necessarily mix in the traditional sense; molecular transport between them must often be through diffusion.
High specificity of chemical and physical properties (concentration, pH, temperature, shear force, etc.) can also be ensured resulting in more uniform reaction conditions and higher grade products in single and multi-step reactions. Effects of micro domain
laminar flow
surface tension
electrowetting
fast thermal relaxation
electrical surface charges
diffusion Key application areas
Microfluidic structures include micropneumatic systems, i.e. microsystems for the handling of off-chip fluids (liquid pumps, gas valves, etc), and microfluidic structures for the on-chip handling of nano- and picolitre volumes. To date, the most successful commercial application of microfluidics is the inkjet printhead. Significant research has been applied to the application of microfluidics for the production of industrially relevant quantities of material.
Advances in microfluidics technology are revolutionizing molecular biology procedures for enzymatic analysis (e.g., glucose and lactate assays), DNA analysis (e.g., polymerase chain reaction and high-throughput sequencing), and proteomics. The basic idea of microfluidic biochips is to integrate assay operations such as detection, as well as sample pre-treatment and sample preparation on one chip.
An emerging application area for biochips is clinical pathology, especially the immediate point-of-care diagnosis of diseases. In addition, microfluidics-based devices, capable of continuous sampling and real-time testing of air/water samples for biochemical toxins and other dangerous pathogens, can serve as an always-on "bio-smoke alarm" for early warning. Continuous-flow microfluidics
These technologies are based on the manipulation of continuous liquid flow through microfabricated channels. Actuation of liquid flow is implemented either by external pressure sources, external mechanical pumps, integrated mechanical micropumps, or by combinations of capillary forces and electrokinetic mechanisms. Continuous-flow microfluidic operation is the mainstream approach because it is easy to implement and less sensitive to protein fouling problems. Continuous-flow devices are adequate for many well-defined and simple biochemical applications, and for certain tasks such as chemical separation, but they are less suitable for tasks requiring a high degree of flexibility or complicated fluid manipulations. These closed-channel systems are inherently difficult to integrate and scale because the parameters that govern flow field vary along the flow path making the fluid flow at any one location dependent on the properties of the entire system. Permanently-etched microstructures also lead to limited reconfigurability and poor fault tolerance capability.
Process monitoring capabilities in continuous-flow systems can be achieved with highly sensitive microfluidic flow sensors based on MEMS technology which offer resolutions down to the nanoliter range. Digital (droplet-based) microfluidics
Alternatives to the above closed-channel continuous-flow systems include novel open structures, where discrete, independently controllable droplets are manipulated on a substrate using electrowetting. Following the analogy of digital microelectronics, this approach is referred to as digital microfluidics, which was pioneered as the "fluid transistor" by Cytonix and subsequently commercialized by Duke University. By using discrete unit-volume droplets, a microfluidic function can be reduced to a set of repeated basic operations, i.e., moving one unit of fluid over one unit of distance. This "digitization" method facilitates the use of a hierarchical and cell-based approach for microfluidic biochip design. Therefore, digital microfluidics offers a flexible and scalable system architecture as well as high fault-tolerance capability. Moreover, because each droplet can be controlled independently, these systems also have dynamic reconfigurability, whereby groups of unit cells in a microfluidic array can be reconfigured to change their functionality during the concurrent execution of a set of bioassays. Although droplets are manipulated in confined microfluidic channels, since the control on droplets is not independent, it should not be confused as "digital microfluidics". One common actuation method for digital microfluidics is electrowetting-on-dielectric (EWOD). Many lab-on-a-chip applications have been demonstrated within the digital microfluidics paradigm using electrowetting. However, recently other techniques for droplet manipulation have also been demonstrated using Surface Acoustic Waves, optoelectrowetting etc. DNA chips (microarrays)
Early biochips were based on the concept of a DNA microarray, e.g., the GeneChip DNAarray from Affymetrix, which is a piece of glass, plastic or silicon substrate on which pieces of DNA (probes) are affixed in a microscopic array. Similar to a DNA microarray, a protein array is a miniature array where a multitude of different capture agents, most frequently monoclonal antibodies, are deposited on a chip surface; they are used to determine the presence and/or amount of proteins in biological samples, e.g., blood. A drawback of DNA and protein arrays is that they are neither reconfigurable nor scalable after manufacture. Digital microfluidics has been described as a means for carrying out Digital PCR. Molecular biology
In addition to microarrays biochips have been designed for two-dimensional electrophoresis, transcriptome analysis, and PCR amplification. Other applications include various electrophoresis and liquid chromatography applications for proteins and DNA, cell separation, in particular blood cell separation, protein analysis, cell manipulation and analysis including cell viability analysis and microorganism capturing. Evolutionary biology
Three Micro Habitat Patches MHPs connected by dispersal corridors (indicated here as Ji,j) into a 1D lattice. The ecosystem service (of habitat renewal) to each MHP represented here as i (red arrows). Each MHP can also hold different carrying capacity Ki for its supporting local population of bacterial cells (depicted in green).
By combining microfluidics with landscape ecology and nanofluidics, a nano/micro fabricated fluidic landscape can be constructed by building local patches of bacterial habitat and connecting them by dispersal corridors. The resulting landscapes can be used as physical implementations of an adaptive landscape , by generating a spatial mosaic of patches of opportunity distributed in space and time. The patchy nature of these fluidic landscapes allows for the study of adapting bacterial cells in a metapopulation system. The evolutionary ecology of these bacterial systems in these synthetic ecosystems allows for using biophysics to address questions in evolutionary biology. Cellular biophysics
By rectifying the motion of individual swimming bacteria , microfluidic structures can be use to extract mechanical motion from a population of motile bacterial cells . This way, bacteria-powered rotors can be built. Optics
Tuneable Microlens Array Acoustic droplet ejection (ADE)
Acoustic droplet ejection uses a pulse of ultrasound to move low volumes of fluids (typically nanoliters or picoliters) without any physical contact. This technology focuses acoustic energy into a fluid sample in order to eject droplets as small as a millionth of a millionth of a liter (picoliter = 10-12 liter). ADE technology is a very gentle process, and it can be used to transfer proteins, high molecular weight DNA and live cells without damage or loss of viability. This feature makes the technology suitable for a wide variety of applications including proteomics and cell-based assays. Fuel cells
For more details on this topic, see Electroosmotic pump.
Microfluidic fuel cells can use laminar flow to separate the fuel and its oxidant to control the interaction of the two fluids without a physical barrier as would be required in conventional fuel cells. See also
Fluidics
Nanofluidics
List of microfluidics research groups
Lab on a chip
Digital microfluidics
Fluids@Home References
^ Kirby, B.J. (2010). Micro- and Nanoscale Fluid Mechanics: Transport in Microfluidic Devices. Cambridge University Press. http://www.kirbyresearch.com/textbook. 
^ Karniadakis, G.M., Beskok, A., Aluru, N. (2005). Microflows and Nanoflows. Springer Verlag. 
^ Bruus, H. (2007). Theoretical Microfluidics. Oxford University Press. 
^ Tabeling, P. (2005). Introduction to Microfluidics. Oxford University Press. 
^ J Shestopalov, J. D. Tice and R. F. Ismagilov,"Multi-step synthesis of nanoparticles performed on millisecond time scale in a microfluidic droplet-based system" Lab Chip, 2004, 4, 316 - 321, DOI: 10.1039/b403378g.
^ Nguyen, N.T., Wereley, S. (2006). Fundamentals and Applications of Microfluidics. Artech House. 
^ Wei Li, Jesse Greener, Dan Voicu and Eugenia Kumacheva "Multiple modular microfluidic (M3) reactors for the synthesis of polymer particles" Lab Chip, 2009, 9, 2715 - 2721, DOI: 10.1039/b906626h.
^ Herold, KE; Rasooly, A (editor) (2009). Lab-on-a-Chip Technology: Fabrication and Microfluidics. Caister Academic Press. ISBN 978-1-904455-46-2. 
^ a b Herold, KE; Rasooly, A (editor) (2009). Lab-on-a-Chip Technology: Biomolecular Separation and Analysis. Caister Academic Press. ISBN 978-1-904455-47-9. 
^ Chang, H.C., Yeo, Leslie (2009). Electrokinetically Driven Microfluidics and Nanofluidics. Cambridge University Press. 
^ Fan et al. (2009). "Two-Dimensional Electrophoresis in a Chip". Lab-on-a-Chip Technology: Biomolecular Separation and Analysis. Caister Academic Press. ISBN 978-1-904455-47-9. 
^ Bontoux et al. (2009). "Elaborating Lab-on-a-Chips for Single-cell Transcriptome Analysis". Lab-on-a-Chip Technology: Biomolecular Separation and Analysis. Caister Academic Press. ISBN 978-1-904455-47-9. 
^ Cady, NC (2009). "Microchip-based PCR Amplification Systems". Lab-on-a-Chip Technology: Biomolecular Separation and Analysis. Caister Academic Press. ISBN 978-1-904455-47-9. 
^ Keymer J.E., P. Galajda, C. Muldoon R., and R. Austin (November 2006). "Bacterial metapopulations in nanofabricated landscapes". PNAS 103 (46): 17290-295. doi:10.1073/pnas.0607971103. 
^ Galajda P, J.E. Keymer, P Chaikin, R. Austin (December 2007). "A Wall of Funnels Concentrates Swimming Bacteria". Journal of Bacteriology 189 (23): 8704-8707. doi:10.1128/JB.01033-07. 
^ Angelani L., R. Di Leonardo, G. Ruocco (2009). "Self-Starting Micromotors in a Bacterial Bath". Phys. Rev. Let. 102: 048104. doi:10.1103/PhysRevLett.102.048104. 
^ Di Leonardo R, L. Angelani , G. Ruocco, V. Iebba, M.P. Conte, S. Schippa, F. De Angelis, F. Mecarini, E. Di Fabrizio (October 2009). "A bacterial ratchet motor". arXiv:Condensed Matter.Statistical Mechanics. 
^ Sokolova A., M.M. Apodacac, B.A. Grzybowskic, I.S. Aransona (December 2009). "Swimming bacteria power microscopic gears". PNAS 107 (3): 969-974. doi:10.1073/pnas.0913015107. 
^ Liquid micro-lens array activated by selective electrowetting on lithium niobate substrates S. Grilli, L. Miccio, V. Vespini, A. Finizio, S. De Nicola, and P. Ferraro Optics Express 16, 8084-8093 (2008). http://dx.doi.org/10.1364/OE.16.008084
^ P. Ferraro, L. Miccio, S. Grilli, A. Finizio, S. De Nicola, and V. Vespini, "Manipulating Thin Liquid Films for Tunable Microlens Arrays," Optics & Photonics News 19, 34-34 (2008) http://www.opticsinfobase.org/abstract.cfm?URI=OPN-19-12-34
^ Water Management in PEM Fuel Cells
^ Building a Better Fuel Cell Using Microfluidics
^ Fuel Cell Initiative at MnIT Microfluidics Laboratory Further reading Review Papers
Whitesides, G. M.; "The origins and the future of microfluidics"; Nature 2006, 442, 368-373. Squires, T. M.; Quake, S. R.; Reviews of Modern Physics 2005, 77, 977-1026. Microfluidics: Fluid physics at the nanoliter scale Books
Herold, KE; Rasooly, A (editor) (2009). Lab-on-a-Chip Technology: Fabrication and Microfluidics. Caister Academic Press. ISBN 978-1-904455-46-2. 
Herold, KE; Rasooly, A (editor) (2009). Lab-on-a-Chip Technology: Biomolecular Separation and Analysis. Caister Academic Press. ISBN 978-1-904455-47-9.  External links
Wikibooks has a book on the topic of
Microfluidics
Biomicrofluidics, an open access, peer reviewed journal published by the American Institute of Physics
MEMSuniverse, a Videos and animations of Microfluidic devices and their applications
Supercool microfluidics - Our understanding of life and technology at extreme temperatures could become clearer thanks to a microfluidic device that studies ice formation reported in Chemical Technology from the Royal Society of Chemistry
From microfluidic applications to nanofluidic phenomena - a Chem Soc Rev themed issue showcasing the latest advances in microfluidic and nanofluidic research, guest edited by Albert van den Berg, Harold Craighead and Peidong Yang. Published by the Royal Society of Chemistry Tutorials and summaries
MIFLUS - Microfluidics Terminology tree
Living La Vida LOC(a): A Brief Insight into the World of "Lab on a Chip" and Microfluidics
- Sacrificial Carbon Pastes and Tapes for the manufacture of Microchannels
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